Journal Article

Vitamin D heritability and effect of pregnancy status in Vervet monkeys (Chlorocebus aethiops sabaeus) under conditions of modest and high dietary supplementation.

December 28, 2015

Vitamin D heritability and effect of pregnancy status in Vervet monkeys (Chlorocebus aethiops sabaeus) under conditions of modest and high dietary supplementation. American Journal of Physical Anthropology, 2015. Geetha Chittoor, 1, 2, †, Nicholas M. Pajewski, 3, † , V. Saroja Voruganti, 1, 2, Anthony G. Comuzzie, 4, 5, Thomas B. Clarkson, 6, Matthew Nudy, 7, 8, Peter F. Schnatz, 7, 8, 9, 10, Jay R. Kaplan, 6 and Matthew J. Jorgensen, 6.

Author Affiliations

1 Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, Kannapolis, NC
2 UNC Nutrition Research Institute, University of North Carolina at Chapel Hill, Chapel Hill, Kannapolis, NC
3 Department of Biostatistical Sciences, Wake Forest School of Medicine, Winston-Salem, NC
4 Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX
5 Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX
6 Department of Pathology, Section of Comparative Medicine, Wake Forest School of Medicine, Winston-Salem, NC
7 Department of Obstetrics and Gynecology, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA
8 Department of Internal Medicine, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA
9 Department of Obstetrics and Gynecology, Reading Hospital, Reading, PA
10 Department of Internal Medicine, Reading Hospital, Reading, PA
† Geetha Chittoor and Nicholas M. Pajewski contributed equally to this work.

Abstract

The two objectives of the current study were to: 1) investigate the genetic contributions to variations in serum vitamin D concentrations under two dietary conditions (a standard monkey biscuit diet vs. a diet designed to model typical American consumption); and 2) explore the interaction of vitamin D with pregnancy status using a cohort of pedigreed female vervet/African green monkeys.This study includes 185 female (≥3.5 years) vervet/African green monkeys (Chlorocebus aethiops sabaeus) from a multi-generational, pedigreed breeding colony. The 25(OH)D3 concentrations were first measured seven to eight weeks after consuming a “typical American” diet (TAD), deriving 37, 18, and 45% of calories from fat, protein sources, and carbohydrates, and supplemented with vitamin D to a human equivalent of 1,000 IU/day. Vitamin D concentrations were assessed again when animals were switched to a low-fat, standard biscuit diet (LabDiet 5038) for 8 months, which provided a human equivalent of approximately 4,000 IU/day of vitamin D. All statistical analyses were implemented in SOLAR.Pregnancy was associated with reduced 25(OH)D3 concentrations. Heritability analyses indicated a significant genetic contribution to 25(OH)D3 concentrations in the same monkeys consuming the biscuit diet (h(2) =0.66, P=0.0004) and TAD (h(2) =0.67, P=0.0078) diets, with higher 25(OH)D3 concentrations in animals consuming the biscuit diet. Additionally, there was a significant genotype-by-pregnancy status interaction on 25(OH)D3 concentrations (P<0.05) only among animals consuming the TAD diet.These results support the existence of a genetic contribution to differences in serum 25(OH)D3 concentrations by pregnancy status and emphasize the role of diet (including vitamin D supplementation) in modifying genetic signals as well as vitamin D concentrations.

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