Journal Articles

Table grape consumption reduces adiposity and markers of hepatic lipogenesis and alters gut microbiota in butter fat-fed mice

September 02, 2015

Table grape consumption reduces adiposity and markers of hepatic lipogenesis and alters gut microbiota in butter fat-fed mice,2 Sep 2015 The Journal of Nutritional Biotechnology, Jessie Baldwin1, Brian Collins1, Patricia G. Wolf, Kristina Martinez, Wan Shen, Chia-Chi Chuang, Wei Zhong, Paula Cooney, Chase Cockrell, Eugene Chang, H. Rex Gaskins, Michael K. McIntosh.

Author Afiliations

  • Department of Nutrition, University of North Carolina at Greensboro (UNCG), Greensboro, NC
  • Carl R. Woese Institute of Genomic Biology & Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, IL
  • Department of Medicine, University of Chicago, Chicago, IL
  • Department of Nutrition, University of North Carolina at Greensboro (UNCG), Greensboro, NC
  • Department of Internal Medicine/Section on Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, NC
  • Center for Translational Biomedical Research, UNCG-NCRC, Kannapolis, NC

Abstract

Our objective was to determine if consuming table grapes reduces adiposity and its metabolic consequences and alters gut microbiota in mice fed a high fat (HF), butter-rich diet. C57BL/6 J mice were fed a low fat (LF) diet or HF diet with 3% or 5% grapes for 11 weeks. Total body and inguinal fat were moderately, but significantly reduced in mice fed both levels of grapes compared to their controls. Mice fed 5% grapes had lower liver weights and triglyceride levels, and decreased expression of glycerol-3-phosphate acyltransferase (Gpat1) compared to the 5% controls. Mice fed 3% grapes had lower hepatic mRNA levels of peroxisome proliferator-activated receptor gamma 2, sterol-CoA desaturase 1, fatty-acid binding protein 4, and Gpat1 compared to the 3% controls. Although grape feeding had only a minor impact on markers of inflammation or lipogenesis in adipose tissue or intestine, 3% grapes decreased the intestinal abundance of sulfidogenic Desulfobacter spp., and the Bilophila wadsworthia-specific dissimilatory sulfite reductase gene (dsrA-Bw), and tended to increase the abundance of the beneficial bacterium Akkermansia muciniphilacompared to controls. Additionally, Bifidobacterium, Lactobacillus, Allobaculum, and several other genera correlated negatively with adiposity. Allobaculum in particular was increased in the LF and 3% grapes groups compared to the HF-fed controls. Notably, grape feeding attenuated the HF-induced impairment in epithelial localization of the intestinal tight junction protein zonula occludens. Collectively, these data indicate that some of the adverse health consequences of consuming a HF diet rich in saturated fat can be attenuated by table grape consumption.

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