Emily F. Warner, Michael J. Smith, Qingzhi Zhang, K. Saki Raheem, David O’Hagan, Maria A. O’Connell, Colin D. Kay (2017). Signatures of anthocyanin metabolites identified in humans inhibit biomarkers of vascular inflammation in human endothelial cells. Molecular Nutrition & Food Research.
Department of Nutrition, Norwich Medical School, Bob Champion Research and Education Building, University of East Anglia, Norwich, United Kingdom
School of Pharmacy, University of East Anglia, Norwich, United Kingdom
School of Biological Sciences, University of East Anglia, Norwich, United Kingdom
School of Chemistry, St. Andrews University, Fife, Scotland, United Kingdom
Department of Life Sciences, Faculty of Science and Technology, University of Westminster, London, United Kingdom
Food Bioprocessing & Nutrition Sciences, Plants for Human Health Institute, North Carolina State University, Kannapolis, United States of America
The physiological relevance of contemporary cell culture studies is often perplexing, given the use of unmetabolized phytochemicals at supraphysiological concentrations. We investigated the activity of physiologically relevant anthocyanin metabolite signatures, derived from a previous pharmacokinetics study of 500 mg 13C5-cyanidin-3-glucoside in 8 healthy participants, on soluble vascular adhesion molecule-1 (VCAM-1) and interleukin-6 (IL-6) in human endothelial cells.
Methods and results
Signatures of peak metabolites (previously identified at 1, 6 and 24 h post-bolus) were reproduced using pure standards and effects were investigated across concentrations ten-fold lower and higher than observed mean ( Conclusion
Signatures of anthocyanin metabolites following dietary consumption reduce VCAM-1 and IL-6 production, providing evidence of physiologically relevant biological activity.