Journal Articles

Quantitative trait loci from two genotypes of oat (Avena sativa L.) conditioning resistance to Puccinia coronata

August 14, 2014

Quantitative trait loci from two genotypes of oat (Avena sativa L.) conditioning resistance to Puccinia coronata Phytopathology. 2014 Aug 14, Babiker EM1, Gordon T, Jackson E, Chao S, Harrison S, Carson M, Obert D, Bonman JM.

USDA-ARS , 1691 S 2700 W , Aberdeen, Idaho

General Mills Inc., Kannapolis, NC

USDA-ARS, Fargo, North Dakota

Louisiana State University, Baton Rouge, Louisiana

USDA-ARS, St. Paul, Minnesota

Limagrain Cereal Seeds, Battle Ground, Indiana

USDA-ARS, Aberdeen, Idaho



Abstract Developing oat cultivars with partial resistance to crown rust would be beneficial and cost-effective for disease management. Tworecombinant inbred line populations were generated by crossing the susceptible cultivar ‘Provena’ with two partially resistant sources, ‘CDC Boyer’ and breeding line 94197A1-9-2-2-2-5. A third mapping population was generated by crossing the partially resistant sources to validate the QTL results. The three populations were evaluated for crown rust severity in the field at Louisiana State University (LSU) in 2009 and 2010 and at the Cereal Disease Laboratory (CDL) in St. Paul, Minnesota in 2009, 2010, and 2011. An iSelect platform assays containing 5744 oat single nucleotide polymorphisms was used to genotype the populations. From the 2009 CDL test, linkage analyses revealed two QTL for partial resistance in the Provena/CDC Boyer population on chromosome 19A. One of the 19A QTL was also detected in the 2009 LSU test. Another QTL was detected in on chromosome 12D in the CDL 2009 test. In the Provena/94197A1-9-2-2-2-5 population, only one QTL was detected on chromosome 13A in the CDL 2011 test. The 13A QTL from the Provena/94197A1-9-2-2-2-5 population was validated in CDC Boyer /94197A1-9-2-2-2-5 population in the CDL 2010 and 2011 tests. Comparative analysis of the significant markers sequences with the rice genome database revealed 15 candidate genes for disease resistance on chromosomes 4 and 6 of rice. These genes could be potential targets for cloning from the two resistant parents.

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