Priming of the antiviral immune transcriptome by a mixed flavonoid-fish oil supplement in adult overweight women. The FASEB Journal. April 2014. Lynn Cialdella-Kam2, David Nieman1, Amy Knab5, R. Andrew Shanely1,Mary Pat Meaney1, Fuxia Jin3, Wei Sha6 and Sujoy Ghosh4.
1Appalachian State University Kannapolis NC United States; 2Case Western University Cleveland OH United States; 3Dole Nutrition Research Laboratory Kannapolis NC United States; 4Duke-NUS Graduate Medical School Signapore Singapore; 5Queens University of Charlotte Charlotte NC United States; 6University of North Carolina at Charlotte Kannapolis NC United States.
Flavonoids and fish oils have anti-inflammatory and immune modulating influences. The purpose of this study was to determine if a mixed flavonoid-nutrient-fish oil supplement (Q-Mix; 1000 mg quercetin, 400 mg isoquercetin, 120 mg EGCG, 220 mg EPA, 180 mg DHA) would alter genomic profiles in overweight, middle-aged women. In this double-blinded placebo-controlled trial, women (n=48; age=56.1±8.3y; BMI=33.2±0.8kg/m2) were randomly assigned to either Q-Mix supplement or placebo for 10 weeks. Whole blood derived mRNA, collected at 0- and 10-wk, was analyzed using Affymetrix U133 Plus 2.0 microarrays. Plasma levels increased with Q-mix supplementation by 388% for quercetin, 95% for EPA, 18% DHA, and 20% DPA. Gene Set Enrichment Analysis revealed upregulation of three pathways in Q-Mix vs. placebo related to interferon-induced antiviral mechanism. Consistent with this finding, over-representation analysis also disclosed that infection, immune cell trafficking, and inflammatory pathways were inhibited in Q-mix compared to placebo. Thus, relative to the placebo, a 10-wk Q-mix supplementation elicited a significant rise in plasma quercetin, EPA, DHA, and DPA and stimulated an antiviral whole-blood transcriptomic response in adult overweight women.