Carlotta Cavicchio, Mascia Benedusi, Erika Pambianchi, Alessandra Pecorelli, Franco Cervellati, Vinno Savelli, Duccio Calamandrei, Emilia Maellaro, Giorgio Rispoli, Emanuela Maioli, Giuseppe Valacchi (2017). Potassium ascorbate with ribose – promising therapeutic approach for melanoma treatment. Oxidative Medicine and Cellular Longevity.
1. Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy;
2. Plants for Human Health Institute, Department of Animal Sciences, North Carolina State University, Kannapolis, NC, USA;
3. Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy;
4. Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Siena, Italy;
5. Department of Molecular and Developmental Medicine , University of Siena, Siena, Italy;
6. Department of Life Sciences, University of Siena, Siena, Italy.
While surgery is the definitive treatment for early-stage melanoma, the current therapies against advanced melanoma do not yet provide an effective, long-lasting control of the lesions and a satisfactory impact on patient survival. Thus, research is also focused on novel treatments that could potentiate the current therapies. In the present study, we evaluated the effect of Potassium Ascorbate with Ribose (PAR) treatment on the human melanoma cell line, A375, in 2D and 3D models. In the 2D model, in line with the current literature, the pharmacological treatment with PAR decreased cell proliferation and viability. In addition, an increase of Connexin 43 mRNA and protein were observed. This novel finding was confirmed in PAR-treated melanoma cells cultured in 3D, where an increase of functional gap junctions and a higher spheroid compactness were observed. Moreover, in the 3D model, a remarkable decrease in the size and volume of spheroids was observed, further supporting the treatment efficacy observed in
the 2D model. In conclusion, our results suggest that PAR could be used as a safe adjuvant approach in
support to conventional therapies for the treatment of melanoma.