Plasma Cholesterol-Lowering Activity of Gingerol- and Shogaol-Enriched Extract Is Mediated by Increasing Sterol Excretion. Journal of Agricultural and Food Chemistry. October 7, 2014. Lin Lei †, Yuwei Liu †, Xiaobo Wang †, Rui Jiao ‡,Ka Ying Ma †, Yuk Man Li †, Lijun Wang †, Sun Wa Man †, Shengmin Sang §, Yu Huang #, and Zhen-Yu Chen *†
† Food and Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
‡ College of Science and Engineering, Jinan University, Guangzhou, China
§Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, North Carolina Research Campus, Kannapolis, North Carolina 28081,United States
# School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
The present study investigated the cholesterol-lowering activity of gingerol- and shogaol-enriched ginger extract (GSE). Thirty hamsters were divided into three groups and fed the control diet or one of the two experimental diets containing 0.5 and 1.0% GSE. Plasma total cholesterol, liver cholesterol, and aorta atherosclerotic plaque were dose-dependently decreased with increasing amounts of GSE added into diets. The fecal sterol analysis showed dietary GSE increased the excretion of both neutral and acidic sterols in a dose-dependent manner. GSE down-regulated the mRNA levels of intestinal Niemann–Pick C1-like 1 protein (NPC1L1), acyl CoA:cholesterol acyltransferase 2 (ACAT2), microsomal triacylglycerol transport protein (MTP), and ATP binding cassette transporter 5 (ABCG5), whereas it up-regulated hepatic cholesterol-7α-hydroxylase (CYP7A1). It was concluded that beneficial modification of the lipoprotein profile by dietary GSE was mediated by enhancing excretion of fecal cholesterol and bile acids via up-regulation of hepatic CYP7A1 and down-regulation of mRNA of intestinal NPC1L1, ACAT2, and MTP.