Journal Articles

RNA-Seq links AINTEGUMENTA and AINTEGUMENTA-LIKE6 to cell wall remodeling and plant defense pathways in Arabidopsis

May 26, 2016

Beth A. Krizek, Carlton J. Bequette, Kaimei Xu, Ivory C Blakley, Zheng Qing Fu, Johannes Stratmann and Ann E. Loraine (2016). RNA-Seq links AINTEGUMENTA and AINTEGUMENTA-LIKE6 to cell wall remodeling and plant defense pathways in Arabidopsis. Plant Physiology, 171(1).

Author Affiliations

University of South Carolina CITY: Columbia STATE: South Carolina POSTAL_CODE: 29208 United States Of America [US] University of South Carolina CITY: Columbia STATE: SC United States Of America [US] University of South Carolina CITY: Columbia STATE: South Carolina United States Of America [US] University of North Carolina at Charlotte CITY: Charlotte STATE: North Carolina United States Of America [US] University of South Carolina CITY: Columbia STATE: South Carolina United States Of America [US] University of South Carolina CITY: Columbia STATE: South Carolina POSTAL_CODE: 29208 United States Of America [US] University of North Carolina at Charlotte CITY: Charlotte STATE: North Carolina POSTAL_CODE: 28223 United States Of America [US]

Abstract

AINTEGUMENTA (ANT) and AINTEGUMENTA-LIKE6 (AIL6) are two related transcription factors in Arabidopsis thaliana that have partially overlapping roles in several aspects of flower development including floral organ initiation, identity specification, growth and patterning. To better understand the biological processes regulated by these two transcription factors, we performed RNA-Seq on ant ail6 double mutants. We identified thousands of genes that are differentially expressed in the double mutant compared to wild type. Analyses of these genes suggest that ANT and AIL6 regulate floral organ initiation and growth through modifications to the cell wall polysaccharide pectin. We find reduced levels of de-methyl-esterified homogalacturonan and altered patterns of auxin accumulation in early stages of ant ail6 flower development. The RNA-Seq experiment also reveals cross-regulation of AIL gene expression at the transcriptional level. The presence of a number of overrepresented GO terms related to plant defense in the set of genes differentially expressed in ant ail6 suggest that ANT and AIL6 also regulate plant defense pathways. Furthermore, we find that ant ail6 plants have elevated levels of two defense hormones: salicylic acid (SA) and jasmonic acid (JA) and show increased resistance to the bacterial pathogen Pseudomonas syringae. These results suggest that ANT and AIL6 regulate biological pathways that are critical for both development and defense.

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