Phytochemical Characterization And Anti-inflammatory Activity Of The Mexican Fruit Styrax ramirezii Greenm, Apr 2015, The FASEB Journal, Jorge Guerrero-Medina2 and Mary Ann Lila1, Michael Timmers Dr1,
- 1PHHI, Department of Food, Bioprocessing and Nutrition Sciences North Carolina State University Kannapolis North Caolina United States
- 2Agrobiologia Department Universidad Michoacana de San Nicolás de Hidalgo Uruapan Michoacán Mexico
With an increasing demand on health care systems due to metabolic syndrome-related conditions, fruits as part of a healthy diet can provide a proactive means to counter the chronic inflammation associated with these diseases. A Mexican species, locally valued as a functional fruit, Styrax ramirezii Greenm, was examined in this study to gauge phytochemical profile and anti-inflammatory activity. Following extraction of the dried drupes with acidified 80% ethanol, it was determined that the total phenolic content of the crude extract was 18 mg/g DW gallic acid equivalents (GAE) using the Folin-Ciocalteu assay. Proanthocyanidin levels were 4.9 mg/g DW as procyanidin A2 equivalents using the DMAC assay and anthocyanin levels were 6.5 mg/g DW as cyanidin-3-glucoside equivalents from HPLC analysis. Like other members of the Styrax family, norneolignans were found to be present, which are known to possess biological activity such as cytotoxicity, anti-bacterial and anti-inflammatory activity. Two major norneolignans were isolated. The crude extract and the norneolignans egonol and homoegonol (all at 50 µg/ml), were found to reduce nitric oxide levels by 90%, 49% and 45% respectively compared to the negative control, suggesting that a combination of inherent phytoactive compounds potentiated bioactivity. The crude extract, egonol and homoegonol were also found to lower ROS presence by 27%, 19% and 18% respectively. These results support the potential for the drupes from S. ramirezii as a rich source of polyphenolic compounds to promote health and help against obesity related inflammation.