Journal Articles

Phytochemical Characterization And Anti-inflammatory Activity Of The Mexican Fruit Styrax ramirezii Greenm

April 07, 2015

Phytochemical Characterization And Anti-inflammatory Activity Of The Mexican Fruit Styrax ramirezii Greenm, Apr 2015, The FASEB Journal, Jorge Guerrero-Medina2 and Mary Ann Lila1, Michael Timmers Dr1,

  1. 1PHHI, Department of Food, Bioprocessing and Nutrition Sciences North Carolina State University Kannapolis North Caolina United States
  2. 2Agrobiologia Department Universidad Michoacana de San Nicolás de Hidalgo Uruapan Michoacán Mexico


With an increasing demand on health care systems due to metabolic syndrome-related conditions, fruits as part of a healthy diet can provide a proactive means to counter the chronic inflammation associated with these diseases. A Mexican species, locally valued as a functional fruit, Styrax ramirezii Greenm, was examined in this study to gauge phytochemical profile and anti-inflammatory activity. Following extraction of the dried drupes with acidified 80% ethanol, it was determined that the total phenolic content of the crude extract was 18 mg/g DW gallic acid equivalents (GAE) using the Folin-Ciocalteu assay. Proanthocyanidin levels were 4.9 mg/g DW as procyanidin A2 equivalents using the DMAC assay and anthocyanin levels were 6.5 mg/g DW as cyanidin-3-glucoside equivalents from HPLC analysis. Like other members of the Styrax family, norneolignans were found to be present, which are known to possess biological activity such as cytotoxicity, anti-bacterial and anti-inflammatory activity. Two major norneolignans were isolated. The crude extract and the norneolignans egonol and homoegonol (all at 50 µg/ml), were found to reduce nitric oxide levels by 90%, 49% and 45% respectively compared to the negative control, suggesting that a combination of inherent phytoactive compounds potentiated bioactivity. The crude extract, egonol and homoegonol were also found to lower ROS presence by 27%, 19% and 18% respectively. These results support the potential for the drupes from S. ramirezii as a rich source of polyphenolic compounds to promote health and help against obesity related inflammation.

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