Ximena Maria Muresan, Claudia Sticozzi, Giuseppe Belmonte, Vinno Savelli, Pablo Evelson, Giuseppe Valacchi (2017). Modulation of cutaneous scavenger receptor B1 levels by exogenous stressors impairs “in vitro” wound closure. Mechanisms of Ageing and Development.
Dept. of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy
Plants for Human Health Institute, Animal Sciences Dept., NC Research Campus, NC State University, NC
Department of Medical, Surgery and Neurosciences, University of Siena, Siena, Italy
Institute of Biochemistry and Molecular Medicine (IBIMOL-UBA-CONICET), Pharmacy and Biochemistry School, University of Buenos Aires, Buenos Aires, Argentina
Scavenger receptor B1 (SR-B1) is a trans-membrane protein, involved in tissue reverse cholesterol transport. Several studies have demonstrated that SR-B1 is also implicated in other physiological processes, such as bacteria and apoptotic cells recognition and regulation of intracellular tocopherol and carotenoids levels. Among the tissues where it is localized, SR-B1 has been shown to be significantly expressed in human epidermis. Our group has demonstrated that SR-B1 levels are down-regulated in human cultured keratinocytes by environmental stressors, such as cigarette smoke, via cellular redox imbalance. Our present study aimed to investigate whether such down-regulation was confirmed in a 3D skin model and under other environmental challengers such as particulate matter and ozone. We also investigated the association between oxidation-induced SR-B1 modulation and impaired wound closure. The data obtained showed that not only cigarette, but also the other environmental stressors reduced SR-B1 expression in epidermal cutaneous tissues and that this effect might be involved in impaired wound healing.