Journal Articles

Entire plastid phylogeny of the carrot genus ( Daucus , Apiaceae): Concordance with nuclear data and mitochondrial and nuclear DNA insertions to the plastid 1

February 23, 2017

David M. Spooner, Holly Ruess, Massimo Iorizzo, Douglas Senalik, and Philipp Simon (2017). Entire plastid phylogeny of the carrot genus (Daucus, Apiaceae): Concordance with nuclear data and mitochondrial and nuclear DNA insertions to the plastid 1. American Journal of Botany 104 (2):1 –17.

Author Affiliations

U.S. Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit
Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, Wisconsin 53706-1590 USA
Plants for Human Health Institute, Department of Horticultural Sciences, North Carolina State University, 600 Laureate Way, Kannapolis, North Carolina 28081 USA.

Abstract

PREMISE OF THE STUDY: We explored the phylogenetic utility of entire plastid DNA sequences in Daucus and compared the results with prior phylogenetic
results using plastid and nuclear DNA sequences.

METHODS: We used Illumina sequencing to obtain full plastid sequences of 37 accessions of 20 Daucus taxa and outgroups, analyzed the data with phylogenetic
methods, and examined evidence for mitochondrial DNA transfer to the plastid ( Dc MP).

KEY RESULTS: Our phylogenetic trees of the entire data set were highly resolved, with 100% bootstrap support for most of the external and many of the
internal clades, except for the clade of D. carota and its most closely related species D. syrticus . Subsets of the data, including regions traditionally used as phylogenetically informative regions, provide various degrees of soft congruence with the entire data set. There are areas of hard incongruence, however,
with phylogenies using nuclear data. We extended knowledge of a mitochondrial to plastid DNA insertion sequence previously named Dc MP and identified the first instance in flowering plants of a sequence of potential nuclear genome origin inserted into the plastid genome. There is a relationship of inverted repeat junction classes and repeat DNA to phylogeny, but no such relationship with nonsynonymous mutations.

CONCLUSIONS: Our data have allowed us to (1) produce a well-resolved plastid phylogeny of Daucus , (2) evaluate subsets of the entire plastid data for
phylogeny, (3) examine evidence for plastid and nuclear DNA phylogenetic incongruence, and (4) examine mitochondrial and nuclear DNA insertion into
the plastid.

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