Journal Articles

Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing

April 07, 2016

Mccallum, S., Graham, J., Jorgensen, L., Rowland, L. J., Bassil, N. V., Hancock, J. F., Wheeler, E. J., Vining, K., Poland, J. A., Olmstead, J. W., Buck, E., Wiedow, C., Jackson, E., Brown, A., Hackett, C. A. (2016). Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing. Molecular Breeding, 36(4).

Author Affiliations

1. Cell and Molecular Science Programme, James Hutton Institute, Dundee, Scotland, UK
2. Genetic Improvement of Fruits and Vegetables Laboratory, USDA-ARS, Beltsville, MD, USA
3. National Clonal Germplasm Repository, USDA-ARS, Corvallis, OR, USA
4. Department of Horticulture, Michigan State University, East Lansing, MI, USA
5. MBG Marketing, Berry Blue, Grand Junction, MI, USA
6. Department of Horticulture, Oregon State University, Corvallis, OR, USA
7. Wheat Genetics Resource Center, Department of Plant Pathology and Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA
8. Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
9. The New Zealand Institute for Plant and Food Research Limited, Private Bag 11600, Palmerston North, 4442, New Zealand
10. General Mills Crop Biosciences, Wheat Innovation Center, Manhattan, KS, 66502, USA
11. Plants for Human Health Institute, Department of Horticultural Science, North Carolina State University, Kannapolis, NC, USA
12. Biomathematics and Statistics Scotland, Dundee, Scotland, UK

Abstract

The construction of the first genetic map in autotetraploid blueberry has been made possible by the development of new SNP markers developed using genotyping by sequencing in a mapping population created from a cross between two key highbush blueberry cultivars, Draper × Jewel (Vaccinium corymbosum). The novel SNP markers were supplemented with existing SSR markers to enable the alignment of parental maps. In total, 1794 single nucleotide polymorphic (SNP) markers and 233 simple sequence repeat (SSR) markers exhibited segregation patterns consistent with a random chromosomal segregation model for meiosis in an autotetraploid. Of these, 700 SNPs and 85 SSRs were utilized for construction of the ‘Draper’ genetic map, and 450 SNPs and 86 SSRs for the ‘Jewel’ map. The ‘Draper’ map comprises 12 linkage groups (LG), associated with the haploid chromosome number for blueberry, and totals 1621 cM while the ‘Jewel’ map comprises 20 linkage groups totalling 1610 cM. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents.

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