Construction of a genetic linkage map of an interspecific diploid blueberry population and identification of QTL for chilling requirement and cold hardiness Molecular Breeding, 10 Aug 2014, Lisa J. Rowland, Elizabeth L. Ogden, Nahla Bassil, Emily J. Buck, Susan McCallum, Julie Graham, Allan Brown, Claudia Wiedow, A. Malcolm Campbell, Kathleen G. Haynes, Bryan T. Vinyard
Beltsville Agricultural Research Center-West, United States Department of Agriculture, Agricultural Research Service, Bldg. 010A, 10300 Baltimore Ave., Beltsville, MD, National Clonal Germplasm Repository, United States, Department of Agriculture, Agricultural Research, Service, 33447 Peoria Road, Corvallis, OR 97333, USA, Department of Horticultural Science, Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC 28081, USA, Department of Biology, Davidson College, Davidson, NC 28035, USA, Department of Genetics, James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK
A genetic linkage map has been constructed from an interspecific diploid blueberry population [(Vaccinium darrowii Fla4B × Vaccinium corymbosum W85-20) F1#10 × V. corymbosum W85-23] designed to segregate for cold hardiness and chilling requirement. The map is comprised of 12 linkage groups (equivalent to the haploid chromosome number of diploid blueberry) and totals 1,740 cM. Included on the map are 265 markers based on simple sequence repeats, expressed sequence tag-polymerase chain reactions, single nucleotide polymorphisms, and randomly amplified polymorphic DNAs. The estimated map coverage is 89.9 %, and the average distance between markers is 7.2 cM. The mapping population was evaluated for 2 years (2009 and 2010) for mid-winter bud cold hardiness and for 3 years (2011–2013) for chilling requirement under controlled conditions. Broad-sense heritability of both cold hardiness and chilling requirement was quite high under these conditions with values of 0.88 and 0.86, respectively. One quantitative trait locus/loci (QTL) for cold hardiness and one for chilling requirement were identified that were consistent over at least 2 years. A second weaker QTL for chilling requirement was detected in only one of the 3 years.