Journal Articles

Cardiovascular Mechanisms of Action of Anthocyanins May Be Associated with the Impact of Microbial Metabolites on Heme Oxygenase-1 in Vascular Smooth Muscle Cells

June 21, 2018

Warner EF1, Rodriguez-Ramiro I2, O’Connell MA3, Kay CD4. Cardiovascular Mechanisms of Action of Anthocyanins May Be Associated with the Impact of Microbial Metabolites on Heme Oxygenase-1 in Vascular Smooth Muscle Cells. Molecules. 2018 Apr 13;23(4). pii: E898. doi: 0.3390/molecules23040898.

Author information

1 Department of Nutrition and Preventative Medicine, Norwich Medical School, Bob Champion Research and Education Building, University of East Anglia, Norwich NR4 7UQ, UK. emily.warner@uea.ac.uk.

2 Department of Nutrition and Preventative Medicine, Norwich Medical School, Bob Champion Research and Education Building, University of East Anglia, Norwich NR4 7UQ, UK. I.Rodriguez-Ramiro@uea.ac.uk.

3 School of Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK. m.oconnell@uea.ac.uk.

4 Department of Nutrition and Preventative Medicine, Norwich Medical School, Bob Champion Research and Education Building, University of East Anglia, Norwich NR4 7UQ, UK. cdkay@ncsu.edu.  (Presently: NC State Plants for Human Health Institute, NC Research Campus, Kannapolis, NC.)

Abstract

Anthocyanins are reported to have cardio-protective effects, although their mechanisms of action remain elusive. We aimed to explore the effects of microbial metabolites common to anthocyanins and other flavonoids on vascular smooth muscle heme oxygenase-1 (HO-1) expression. Thirteen phenolic metabolites identified by previous anthocyanin human feeding studies, as well as 28 unique mixtures of metabolites and their known precursor structures were explored for their activity on HO-1 protein expression in rat aortic smooth muscle cells (RASMCs). No phenolic metabolites were active when treated in isolation; however, five mixtures of phenolic metabolites significantly increased HO-1 protein expression (127.4-116.6%, p ≤ 0.03). The present study demonstrates that phenolic metabolites of anthocyanins differentially affect HO-1 activity, often having additive, synergistic or nullifying effects.

KEYWORDS:

anthocyanin; antioxidant; atherosclerosis; metabolism; smooth muscle cells

 

Comments are closed.

Connect With Us