Application of 1H NMR Profiling To Assess Seed Metabolomic Diversity. A Case Study on a Soybean Era Population, 2015 May 5, J Agric Food Chem., Harrigan GG1, Skogerson K1, MacIsaac S2, Bickel A1, Perez T1, Li X1.
- 1†Monsanto Company, 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
- 2§150 North Research Campus Drive, Kannapolis, North Carolina 28081, United States.
1H NMR spectroscopy offers advantages in metabolite quantitation and platform robustness when applied in food metabolomics studies. This paper provides a 1H NMR-based assessment of seed metabolomic diversity in conventional and glyphosate-resistant genetically modified (GM) soybeanfrom a genetic lineage representing ∼35 years of breeding and differing yield potential. 1H NMR profiling of harvested seed allowed quantitation of 27 metabolites, including free amino acids, sugars, and organic acids, as well as choline, O-acetylcholine, dimethylamine, trigonelline, and p-cresol. Data were analyzed by canonical discriminant analysis (CDA) and principal variance component analysis (PVCA). Results demonstrated that 1HNMR spectroscopy was effective in highlighting variation in metabolite levels in the genetically diverse sample set presented. The results also confirmed that metabolite variability is influenced by selective breeding and environment, but not genetic modification. Therefore, metabolite variability is an integral part of crop improvement that has occurred for decades and is associated with a history of safe use.