Journal Articles

A new genetic linkage map of barley (Hordeum vulgare L.) facilitates genetic dissection of height and spike length and angle

August 23, 2013

A new genetic linkage map of barley (Hordeum vulgare L.) facilitates genetic dissection of height and spike length and angle. Field Crop Research. August 23, 2013. Emir Islamovic, Donald E. Obert, Rebekah E. Oliver, Juliet M. Marshall, Kelci J. Miclaus, An Hang, Shiaoman Chao, Gerard R. Lazo, Stephen A. Harrison, Amir Ibrahim, Eric N. Jellen, Peter J. Maughan, Ryan H. Brown, Eric W. Jackson.

USDA-ARS, Aberdeen, ID; General Mills, Inc., NC Research Campus; Limagrain Cereal Seeds, Battle Ground, IN;  SAS Institute Inc., Cary, NC; Brigham Young University, Department of Plant and Wildlife Sciences, Provo, UT; USDA-ARS, Fargo, ND; USDA-ARS, Albany, CA; Louisiana State University, School of Plant, Environmental and Soil Sciences, Baton Rouge, LA; Department of Soil and Life Sciences, Texas A&M University, College Station, TX ; University of Idaho, Department of Plant, Soil, and Entomological Sciences, Idaho Falls, ID;  Department of Plant Sciences, North Dakota State University, Fargo, ND.

Abstract

Plant height and spike length and angle are important agronomic traits in the production of barley (Hordeum vulgare L.) due to strong correlations with lodging and disease. The objective of this study was to use QTL analysis to identify genetic regions associated with each trait in a recombinant inbred line (RIL) mapping population derived from a cross of Falcon by Azhul. Falcon is a spring six-row hulless feed barley with long spikes displaying obtuse angles, while Azhul is a spring dwarf, six-row hulless food barley with short spikes displaying acute angles. The population was genotyped using SNP, DArT and SSR markers and quantitative trait loci (QTL) were detected on chromosomes 2H (102.8 cM, spike length), 3H (89.2 cM, plant height and 38.2, spike angle and length), 4H (19.0 cM, spike length), and 5H (106.7 cM, spike angle). In conclusion, we developed a barley genetic map, which incorporated SNP, DArT and SSR markers, for detection of height and spike length and angle QTL. Three spike angle, one spike length and one plant height QTL were novel and by using comparative genomics we identified possible candidate genes involved in gibberellic acid signaling and auxin- and ethylene-responsive pathways. This knowledge can be used to generate suitable markers for barley breeding improvement.

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