Journal Articles

A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter

June 21, 2018

Zeng X1, Tang R1, Guo H1, Ke S1, Teng B2, Hung YH3,4, Xu Z1, Xie XM1, Hsieh TF5,6, Zhang XQ7. A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter. Plant Mol Biol. 2017 May;94(1-2):137-148. doi: 10.1007/s11103-017-0598-4.

Author information

1 Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.

2 Anhui Provincial Key Laboratory of Rice Genetics and Breeding, Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.

3 Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, 28081, USA.

4 Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, 27695, USA.

5 Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, 28081, USA. thsieh3@ncsu.edu.

6 Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, 27695, USA. thsieh3@ncsu.edu.

7 Guangdong Engineering Research Center of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. aacrav@163.com.

Abstract

A wide range of molecules are transported across membranes by the ATP binding cassette (ABC) transporters. Plants possess a collection of ABC proteins bearing similarities to the components of prokaryotic multi subunit ABC transporters, designed as ABC group I. However the functions of most of them are not well understood. Here, we characterized a naturally occurring rice mutant that exhibited albino phenotype under continuous rainy days in the field, but gradually recovered to normal green after the rainy season. Molecular and genetic analyses revealed that the phenotypes were caused by a mutation in the OsABCI8 that encoded a member of the ABCI family. Subcellular localization demonstrated that OsABCI8 is a chloroplast ABC transporter. Expression of OsABCI8 is significantly enhanced in rainy days compared to sunny days. Besides defects in chloroplast development and chlorophyll biosynthesis, the mutant phenotype is accompanied by a higher accumulation of iron, suggesting that OsABCI8 is involved in iron transportation and/or homeostasis in rice. Our results demonstrate that OsABCI8 represents a conserved ABCI protein involved in transition metals transportation and/or homeostasis and suggest an important role of the plastid-localized OsABCI8 for chloroplast development.

KEYWORDS:

ABC transporter; Chloroplast development; Iron transport; Rice

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