Journal Articles

Comparative Systems Biology Reveals Allelic Variation Modulating Tocochromanol Profiles in Barley (Hordeum vulgare L.)

May 12, 2014

Comparative Systems Biology Reveals Allelic Variation Modulating Tocochromanol Profiles in Barley (Hordeum vulgare L.) PLoS One. May 12,2014; eCollection 2014. Oliver RE1, Islamovic E2, Obert DE3, Wise ML4, Herrin LL4, Hang A2, Harrison SA5, Ibrahim A6, Marshall JM7, Miclaus KJ8, Lazo GR9, Hu G2, Jackson EW10.

1Department of Plant Sciences, North Dakota State University, Fargo, North Dakota, United States of America, formerly USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America; 2USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America; 3Limagrain Cereal Seeds, Battle Ground, Indiana, United States of America, formerly USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America; 4USDA-ARS, Cereal Crops Research Unit, Madison, Wisconsin, United States of America;5School of Plant, Environmental and Soil Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America; 6Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas, United States of America; 7Department of Plant, Soil, and Entomological Sciences, University of Idaho Research and Extension, Idaho Falls, Idaho, United States of America; 8SAS Institute Inc., JMP Genomics Development, Cary, North Carolina, United States of America; 9USDA-ARS, Western Regional Research Center, Genomics and Gene Discovery, Albany, California, United States of America; 10Crop Biosciences, General Mill, Inc., Kannapolis, North Carolina, United States of America, formerly USDA-ARS, Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho, United States of America.

Abstract

Tocochromanols are recognized for nutritional content, plant stress response, and seed longevity. Here we present a systems biological approach to characterize and develop predictive assays for genes affecting tocochromanol variation in barley. Major QTL, detected in three regions of a SNP linkage map, affected multiple tocochromanol forms. Candidate genes were identified through barley/rice orthology and sequenced in genotypes with disparate tocochromanol profiles. Gene-specific markers, designed based on observed polymorphism, mapped to the originating QTL, increasing R2 values at the respective loci. Polymorphism within promoter regions corresponded to motifs known to influence gene expression. Quantitative PCR analysis revealed a trend of increased expression in tissues grown at cold temperatures. These results demonstrate utility of a novel method for rapid gene identification and characterization, and provide a resource for efficient development of barley lines with improved tocochromanol profiles.

PMID: 24820172 [PubMed – in process] PMCID: PMC4018352

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