David H. Murdock Research Institute

GCxGC-MS Validated for Metabolomics Profiling, Biomarker Discovery

November 19, 2015

Read the story on Advanced Health Network.

Scientists at the David H. Murdock Research Institute (DHMRI), located at the NC Research Campus in Kannapolis, NC, validated the use of comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS) for metabolite biomarker discovery.

“GC x GC-MS is an approach to biomarker research that tends to be under-used,” commented Kevin Knagge, PhD, group leader of the DHMRI Analytical Sciences Laboratory (ASL), which includes metabolomics, NMR and proteomics. “The advantage we found is that it increases the number of compounds that can be seen, greatly improving global metabolic profiling.”

DHMRI scientists tested 109 human serum samples with GCxGC-MS and compared them to the standard approach of chromatography time-of-flight mass spectrometry (GC-MS). Using GCxGC-MS more than two times as many metabolites were identified. In addition, the GCxGC-MS analysis identified 34 metabolites with statistically significant differences compared to 23 in the GC-MS analysis when associated to controls. The study results are detailed in the Journal of Proteome Research in the paper entitled “Comparison of GC-MS and GCxGC-MS in the Analysis of Human Serum Samples for Biomarker Discovery.”

Working with Xiang Zhang, PhD, at the University of Louisville, Kentucky, a specialized software program was developed to streamline the analysis of the GCxGC-MS.

“Combining the new software program and the technology that we have on our instrument allowed us to do more elaborate data analysis and data collections, which then increased the resolution and separation,” Knagge said. “We want more metabolomics data for our global metabolomics profiling and for the studies our collaborators bring to us, and this new methodology does that.”

Beyond serum, GCxGC-MS can profile metabolites in urine or tissue samples as well as plant materials. DHMRI is currently using GCxGC-MS for metabolomics profiling and biomarker research related to the prevention and treatment of chronic diseases.

DHMRI is located on the NC Research Campus in Kannapolis, NC and is dedicated to collaborating with companies, institutions and researchers throughout the world to make food nutritious, therapies effective, prevention possible and people healthier by applying a multidisciplinary approach to science that integrates genomics, analytical sciences, cellular sciences and bioinformatics. To learn more, visit http://www.dhmri.org.

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